Multi-wavelength fluorescence polarization immunoassays for simultaneous detection of amantadine and ribavirin in chicken and human serum

In this study, a homologous multi-wavelength fluorescence polarization immunoassays (MWFPIA) for rapid determination of amantadine (AMD) and ribavirin (RVB) simultaneously was established. The MWFPIA could quantify AMD RVB concentration in 1 min. After optimisation, long-wavelength tracers AEDA-AF647 with monoclonal antibody (Mab) 1A12 RVB-CP-EDF polyclonal (Pab) G412 were chosen the development MWFPIA. limit detections (LODs) 1.7 1.0 μg/kg chickens, 17.6 10.4 μg/L human serum, respectively. Recoveries chickens 61.2–94.9% 87.3–90.2%, respectively coefficient variations (CVs) lower than 16.7%. For recoveries 60.2–84.3% 63.5–74.2% RVB, respectively, CVs 15.3%. results indicated that be used monitoring antiviral drugs short analysis time, great accuracy high throughput.